【Abstract】 Objective To observe the life cycle of  Monopsyllus anisus (Rothschild, 1907) in the laboratory.  Methods M.anisus （6♂♂, 7♀♀） collected from  Rattus  norvegecus in Urumqi were fed with rats, at 24-26 ℃ and RH 80%-90%. The life cycle of fleas was observed by two methods such as common breeding and culture plate rearing. Results The life cycle from parent oviposition to the oviposition of filial generation adults was 20-25 d, including 4 d of egg stage, 8-10 d of larva stage, 5-8 d of pupa stage and pregnancy 3 d.  Conclusion The life cycle of M.anisus was within 20-25 d in the laboratory, which was similar to the report before.